Water Quality Testing III: Multiplexed PCR for Testing Water Bacteria

Description

Edvotek™ Water Quality Testing III: Multiplexed PCR for Testing Water Bacteria

Includes: instructions, PCR EdvoBeads™, Universal DNA Buffer, LyphoPrimer™ Mix, EdvoQuick™ DNA Ladder, LyphoControl™ (Complete PCR Control), TE Buffer, Proteinase K, Potassium Acetate, DNA Extraction Buffer, BactoBeads™, UltraSpec-Agarose™, Electrophoresis Buffer (50X), SYBR™ Safe Stain, FlashBlue™ Stain, Microcentrifuge & PCR Tubes

Requires : Thermal cycler; waterbath, electrophoresis tank; power supply; micropipets to measure between 5 and 50μL; tips and microcentrifuge

Description

• Drinking water is routinely tested for contamination. If a screening tests positive, more sophisticated tests are required to determine whether harmful microbes are present
• Students use PCR in multiplex format
• Single PCR test detects presence of three separate, classroom-safe organisms
• Analyzed on agarose gels

Experiment times:

• Setup: 60 minutes
• PCR: 120 minutes or overnight
• Electrophoresis: 45 minutes